![]() The experiments were approved by the Committee of Kyoto University as Recombinant DNA Experiments (#190366). This TERT-hMSC were generously gifted from Toguchida Lab., Center for iPS cell Research and Application (CiRA), Kyoto University. The presence and activity of the gene were confirmed by reverse transcription polymerase chain reaction. ![]() Briefly, to make bone marrow stroma-derived mesenchymal stem cell line immortal, TERT and human papilloma virus E6/E7 were transduced by retrovirus-mediated gene transfer. ![]() Preparation of cells and cell sheetsīecause of its proliferative capacity, we used immortalized human mesenchymal stem cell by transduction of telomerase reverse transcriptase (TERT) gene (TERT-hMSC) to generate cell sheets mimicking cardiac tissue sheets which we would like to apply for clinical use in the future. In the present study, we developed a novel endoscopic cell sheet delivery device for minimally-invasive surgical procedure, and validated the feasibility of the procedures of epicardial cell sheet delivery using three-dimensional (3D) printed simulators.Ģ.3. In addition, minimally-invasive surgical procedure without full sternotomy, would reduce risks for future surgical interventions such as heart transplantation by avoiding tissue adhesion. Considering possible unavoidability of immunosuppressants due to allogeneic stem cell transplantation, providing minimally-invasive surgical procedures is mandatory to reduce the possibility of infections after surgery to standardize stem cell sheet transplantation therapies. On the other hand, minimally invasive cardiac surgery with small skin incisions and limited surgical exposure is reported to associate with less surgical site infection. In general, mediastinitis after open heart surgery occurs in 1.5–2% of patients, , ], and the incidence is reported to be higher in heart transplantation patients under administration of immunosuppressants. ![]() Previously, we have reported therapeutic potential of cardiac tissue sheets generated from differentiated cardiovascular lineage cells from mouse embryonic stem cells or human induced pluripotent stem cells for animal heart disease models as pre-clinical studies.įor the transplantation of cell sheet products, median sternotomy or lateral thoracotomy would be required to approach broad region of heart surface. Among them, cell sheet products are considered to hold higher efficiency of grafted cell survival compared to that in direct injection of cell suspension to the heart by virtue of preserved extracellular matrix proteins. To deliver these stem cell products for heart diseases, trans-coronary injection, epicardial injection and cell sheet technique have been introduced. bone marrow-derived hematopoietic stem or stromal cells, skeletal myoblasts, pluripotent stem cells) are being increasingly attempted to be applied for clinical use worldwide, ,, , ]. Stem cell products manufactured from various stem cell populations (e.g. ![]()
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